目的 运用超高效液相色谱-四极杆飞行时间质谱(ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry,UHPLC-Q-TOF-MS)技术比较当归饮片与配伍后当归建中汤标准汤中有机酸、苯酞和香豆素3类成分的组分变化。方法 采用Thermo Accucore C18色谱柱(2.1 mm×150 mm,2.6 μm),质量分数0.1%乙酸-水(A相)-乙腈(B相)作为流动相,电喷雾质谱法(ESI-MS)检测采用正、负离子模式下进行梯度洗脱。应用PeakView软件对当归饮片与当归建中汤中的当归化学成分进行鉴定并记录离子峰强度,以离子峰强度作为成分含量比较的依据。结果 共指认出44个化合物,单味药中有1种成分在当归建中汤中未能检测出,当归配伍后,共有成分中9种含量无差异,5种含量减少,29种含量增加。有机酸类成分中,酸性较强的成分含量下降,其他成分含量增加;香豆素类成分的含量均升高;苯酞类成分的含量变化趋势并无一致性。结论 当归在配伍后多数成分含量增加(除某些成分也有其他组方药材贡献的情况外),与胶体溶液助溶或其他成分增溶作用相关; 绿原酸、阿魏酸等酸性较强有机酸类成分的减少可能与有机酸类溶出竞争有关。
Abstract
OBJECTIVE To compare the composition changes of organic acid, benzophthalide and coumarin in Angelica Sinensis Radix pieces and Danggui jianzhong decoction by UHPLC-Q-TOF-MS. METHODS The column was Thermo Accucore C18 (2.1 mm×150 mm, 2.6 μm),the mobile phase was 0.1% acetic acid-water (phase A)-acetonitrile (phase B). The chemical components of Angelicae sinensis in Angelicae Sinensis Radix pieces and Danggui jianzhong decoction were identified by PeakView, and the ion peak intensity was recorded. The ion peak intensity was used as the basis for the comparison of component content. RESULTS A total of 44 compounds were identified,and one of the compounds could not be detected in Danggui jianzhong decoction. After the compatibility of Angelica sinensis, there was no difference in the content of 9 components,5 decreased and 29 increased. Among the organic acids,the content of components with strong acidity decreased and the content of other components increased. The contents of coumarins increased. There was no consistency in the content change trend of phthalide components. CONCLUSION The content of most components of Angelica sinensis increased after compatibility,which was related to the solubilization of colloidal solution or the solubilization of other components,except that some components also contributed to other prescriptions. The decrease of organic acids with strong acidity such as chlorogenic acid and ferulic acid may be related to the dissolution competition of organic acids.
关键词
当归 /
当归建中汤 /
有机酸 /
香豆素 /
苯酞 /
含量变化 /
超高效液相色谱-四级杆飞行时间质谱
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Key words
Angelica sinensis /
Danggui jianzhong decoction /
organic acid /
coumarin /
benzophthalide /
content change /
UHPLC-Q-TOF-MS
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脚注
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基金
国家自然科学基金项目资助(82104358);河北省重点研发计划项目资助(20372502D,21372503D);河北省自然科学基金面上项目资助(H2021423066);河北中医学院科技能力提升项目资助(KTY2019063);河北中医学院大学生创新项目资助(202114432076,202114432171)
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